Sequencing of Gliotoxin Genes in Clinical and Environmental Aspergillus fumigatus isolates in Iraq

Authors

  • Russel S. Abdulhadi
  • Nemat J. Abdulbaqi

DOI:

https://doi.org/10.24126/jobrc.2018.12.1.546

Keywords:

Aspergillus fumigatus, gliotoxin, PCR Polymerase Chain Reaction, bp base pairs, DNA Deoxyribonucleic Acid

Abstract

Gliotoxin is an important virulence factors in Aspergillus fumigatus. The biosynthesis of this mycotoxin is regulated and expressed by the presence of gliP genes. This study aimed to identify Aspergillus fumigatus isolates in clinical and environmental sources with glip genes using conventional PCR and sequence. To achieve this, DNA was isolated from twenty A. fumigatus isolates using commercial kit. The range of the DNA extracted was 65-210 ng/μl with a purity of 1.5-1.9. Species identification of the A. fumigatus isolates was achieved to a high specificity by using tailored primer. The results showed that all isolates had positive results to the primer and all isolates were able to produce gliotoxin. PCR detected the gliotoxin genes, glip in five isolates. The five PCR product samples were sent for sequence analysis and 25 µl (10 pmol) from the forward primer. The results of all the samples  indicated have a  single band of the desired product of gliP gene of A.fumigatus and the samples sent for sequencing related to molecular weight 190 bp.

Downloads

Published

2018-01-01

How to Cite

Abdulhadi, R. S. ., & Abdulbaqi, N. J. . (2018). Sequencing of Gliotoxin Genes in Clinical and Environmental Aspergillus fumigatus isolates in Iraq . Journal of Biotechnology Research Center, 12(1), 33–39. https://doi.org/10.24126/jobrc.2018.12.1.546

Issue

Section

Research articles