Genotype detection of fimH gene of Acinetobacter baumannii isolated from different clinical cases

Abstract

Background: Forty isolates were obtained of Acinetobacter baumannii from (200) samples, the samples were collected from different cases including: - wounds, burns, Stool, Urinary tract infection urine.

Aim of study: The aim of study is to isolate and diagnose Acinetobacter baumanniii from different clinical cases and to investigate the fimH gene.

Objective: Respiratory tract infection and blood sample.For the period between 1/9/2016 to 30/11/2016.

Materials and methods: Identification of 40 isolates confirmed to be Acinetobacter baumannii included 9 isolates from blood, 1 isolate from urinary tract infections, 4 isolates from each of wound infections and Respiratory tract infection, 8 isolates from burns and 14 isolates from stool sample.

Results: The result revealed that the fimH gene was present in (20) isolates (50%) of Acinetobacter baumannii. The result Showed (7 (isolate (17.5%) of Stool and blood has fimH gene to each of them and Burns (3) isolates (7.5%) has fimH gene and (2) isolate (5%) of respiratory tract infection has fimH gene and finally only (1) isolate (2.5 %) of Wound infection. While all isolated for each of the urinary tract infections doesn't the fimH gene. The gel electrophoresis showed that the molecular weight of fimH gene was 508 bp.

Conclusion:   Sequential analysis detection of fimH showed three silent mutations that did not affect the amino acid translation.