The effect of Alloxan induced diabetes on baseline comet test in male laboratory mice fed on different feeding systems

Abstract

Background: The comet analysis (electromagnetic relay analysis of a single cell) is a
highly sensitive method for determining DNA damage because to exposure to
carcinogens and other substances that consequently affect fertility.
Objective: the aim of this study is to Evaluation the broken DNA in male diabetic
mice. Created by alloxan and knowledge of the effect of some nutritional systems on
the treatment and repair of genetic material due to diabetes.
Materials and methods: In this study were used, 42 male Albino mice labs at the age
of 2-3 months and weight (25-30) g then 6 animals were isolated to represent the
control group, The remaining animals were injected between the thighs by alloxan 150
mg / kg and left the next day to make sure hit by diabetes and randomly distributed to
six groups in addition to a set of Not affected control transactions.
Results and conclusion: It was found from the results of the statistical analysis shown
in figure(1) showed the presence of the highest percentage of injuries in the genetic
material that was in the group of animals with diabetes and untreated for the duration
of the experiment, as it was clear from the photos of this group that the tail length
resulting from the migration of the genetic material DNA to outside the nucleus
because to its damage, as well as the results showed that the group of animals affected
by diabetes and the treatment by adding the Salvia officinalis plant to both plant and
animal nutrition had a significant decrease (<0.05> P) in the proportion of genetic
material injuries where the proportion of high (long guilt) infections was low in these
two The two groups compared to the group of animals with diabetes and untreated for
the duration of the experiment.