The effect of silver nanoparticles prepared from the aqueous extract of the fenugreek plant on some of the hormones associated with polycystic ovary syndrome in female laboratory rats

Abstract

Background: This study dealt with an assessment of the effect of the nanoparticles of the fenugreek plant and the metformin of 50 mg/kg on female laboratory rats infected with the updated ovarian cyst syndrome using the letrazole with a concentration of 1 mg/kg of body weight (except for the healthy group), as ovarian affiliation is a common endocrine disorder that affects 3-13% of women at the age of pregnancy and childbearing. Objective: to assess the effectiveness of medicinal plants represented by the Fenugreek extract with nanoparticles and its ability to treat female rats infected with ovarian cyst syndrome. Materials and Methods: Twenty-four animals of white laboratory rats were tested, and the groups were randomly divided into the following ways: 1- The control group. 2- A group of animals infected with ovaries. 3- A group of animals with cysts, was given orally the nano-fenugreek extract 2 mg/ kg. 4- A group of animals infected with cysts and treated by adding metformin 50 mg/ kg of body weight. After the end of the experiment period (30 days), the blood was withdrawn using a dispensed infection with cardiovis to conduct laboratory tests for hormones that are affected by the new disease, such as Lutein hormone, Follicle-stimulating hormone, Prolactin, Progesterone, and Estrogen. Results showed the following: 1- The injection of litrazole 1 mg/ kg led to an ovarian cyst and negative effects on the animal represented in a moral increase in levels, of lutein hormone and prolactin, while the injury led to a moral decrease in the hormone concentration that stimulates follicles, progesterone, and estrogen. 2- The treatment of an oral dosage of 2 mg/ kg of body weight with the nanoparticle fenugreek extract led to positive effects in the body's hormones represented in a moral increase in FSH, progesterone, estrogen while the same treatment led to a moral decrease in the lutein hormone and prolactin in the body at a level (P> 0.05). For follicles and decrease in prolactin and lutein hormone in the body at the probability level (P> 0.05). Conclusion: The current study has found that the Nano - extract fenugreek can be prepared with the Green synthesis by the use of Nanoscopic silver particles, which is an effective treatment to reduce the strikes in the hormones of the body with ovarian stinging syndrome, as a result of the small surface area to the size of the nanoparticles, so the absorption of cells has more efficient and faster than The normal size of the plant.