The effect of calcium antagonists on the growth of Entamoeba histolytica in vitro

Zahra’a Abdul-Raheem  Ahmed; Ali H.  Ad’hiah; Amna N.  Jasim

doi:10.24126/jobrc.2010.4.1.82

The effect of calcium antagonists on the growth of Entamoeba histolytica in vitro

Authors

Zahra’a Abdul-Raheem Ahmed
Ali H. Ad’hiah
Amna N. Jasim

DOI:

https://doi.org/10.24126/jobrc.2010.4.1.82

Abstract

he E. histolytica parasite was maintained in vitro using Locke-egg medium (LEM) and Liver infusion agar medium (LIAM). The effect of two calcium antagonists (Nifedipine and Ethylene-diaminetetraacetic acid EDTA) on the growth and activity of the parasite in the two culture media was investigated. The calcium antagonists Nifedipine and EDTA inhibited the reproduction rate of E. histolytica in a concentration-dependent manner. For Nifedipine, a concentration of 41.6 mg/ml inhibited the reproduction rate to 99.7% in both media. The EDTA had an approximate effect (98.2 and 95.8)% at a concentration of 0.83 mg/ml in LEM and LIAM media, respectively. Additionally, some cases of a parasite encystment were observed in LEM medium that was treated with Nifedipine.

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Published

2010-01-01

How to Cite

Ahmed, Z. A.-R. ., Ad’hiah, A. H. ., & Jasim, A. N. . (2010). The effect of calcium antagonists on the growth of Entamoeba histolytica in vitro. Journal of Biotechnology Research Center (JOBRC), 4(1), 5–10. https://doi.org/10.24126/jobrc.2010.4.1.82

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Issue

Vol. 4 No. 1 (2010): Issue 1

Section

Research articles

License

This is an Open Access article distributed under the terms of the creative commons Attribution (CC BY) 4.0 license which permits unrestricted use, distribution, and reproduction in any medium or format, and to alter, transform, or build upon the material, including for commercial use, providing the original author is credited.